TWO HYBRID ASSAY Meaning and
Definition
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The Two-Hybrid Assay is a molecular biology technique used to study protein-protein interactions. It is based on the reconstitution of a functional transcription factor by bringing together two separate proteins of interest, one acting as the DNA-binding domain (DBD) and the other as the activation domain (AD). The principle of this assay involves splitting a transcription factor into two separate fragments, both of which are non-functional on their own. The two proteins of interest, when fused to these fragments, are able to interact and reconstitute a functional transcription factor, resulting in the activation of a reporter gene.
To perform the Two-Hybrid Assay, the proteins of interest are fused to the DBD and AD fragments, respectively. These fusion proteins are then co-expressed in a host cell along with a reporter gene controlled by a promoter recognized by the reconstituted transcription factor. If the proteins of interest interact, they bring the DBD and AD fragments closer, enabling the transcription factor to bind to the promoter and activate gene expression. This leads to the detection of a measurable output, such as the production of a fluorescent protein, luminescence, or growth on a selective medium.
The Two-Hybrid Assay has been widely used to explore protein-protein interactions and identify novel interaction partners in various organisms. It provides a powerful tool for studying protein function, characterizing signaling pathways, and investigating protein networks. It allows researchers to gain insights into the diverse biological processes and molecular interactions that occur within cells.
Common Misspellings for TWO HYBRID ASSAY
- rwo hybrid assay
- fwo hybrid assay
- gwo hybrid assay
- ywo hybrid assay
- 6wo hybrid assay
- 5wo hybrid assay
- tqo hybrid assay
- tao hybrid assay
- tso hybrid assay
- teo hybrid assay
- t3o hybrid assay
- t2o hybrid assay
- twi hybrid assay
- twk hybrid assay
- twl hybrid assay
- twp hybrid assay
- tw0 hybrid assay
- tw9 hybrid assay
- two gybrid assay
- two bybrid assay
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