PROTOZOA DRUG SENSITIVITY TESTS Meaning and
Definition
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Protozoa drug sensitivity tests refer to laboratory procedures that are conducted to evaluate the effectiveness of various drugs or medications against protozoa, which are single-celled microorganisms. These tests are used to determine the susceptibility of protozoa to specific drugs and guide the selection of appropriate treatment strategies for protozoal infections.
During these tests, samples of protozoa are obtained from infected individuals or artificially cultured in the laboratory. These samples are then exposed to different drugs or antimicrobial agents, either individually or in combinations. The aim is to assess the response of the protozoa to the drugs and determine their sensitivity or resistance.
Various techniques are employed to carry out protozoa drug sensitivity tests, including the broth microdilution method, disk diffusion method, and the E-test. The broth microdilution method involves exposing the protozoa to different dilutions of drugs in liquid culture medium, and then measuring the growth or inhibition of the protozoa over a specific period. The disk diffusion method is based on the diffusion of drugs from a paper disc placed on a culture medium, where the zone of inhibition around the drug disc indicates its effectiveness. The E-test involves the use of a plastic strip impregnated with a gradient concentration of the drug, which helps determine the minimum inhibitory concentration required to inhibit protozoal growth.
These tests provide valuable information to healthcare professionals regarding protozoa drug resistance patterns, helping them make informed decisions about the most appropriate drugs to treat protozoal infections. By identifying the most effective drugs for an individual patient, protozoa drug sensitivity tests can improve treatment outcomes and reduce the risk of treatment failure or disease recurrence.
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