BAMI ENDONUCLEASE Meaning and
Definition
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BamHI endonuclease is a type II restriction enzyme that is commonly used in molecular biology techniques. It is derived from the bacterium Bacillus amyloliquefaciens strain H. This endonuclease recognizes a specific DNA sequence and cleaves it at specific sites, allowing for controlled manipulation of DNA molecules.
The BamHI endonuclease specifically recognizes and cuts DNA at the palindromic sequence 5'->G/GATCC<-3'. The recognition site contains two symmetrical halves that read the same from 5' to 3' on both strands of DNA. The endonuclease cleaves the DNA exactly between the second G and the first A, resulting in cohesive (sticky) ends with an overhang of 5' overhang of GATC. These cohesive ends facilitate the joining of DNA fragments with complementary ends from different sources, a process known as DNA ligation.
BamHI endonuclease is widely used in recombinant DNA technology for the precise insertion of a DNA fragment into a vector or genome. Its ability to generate cohesive ends simplifies the creation of recombinant DNA molecules. The enzyme is also a valuable tool in DNA analysis techniques such as restriction fragment length polymorphism (RFLP) analysis, which helps identify genetic variations and analyze DNA samples.
Overall, BamHI endonuclease plays a crucial role in molecular biology experiments and research, enabling the manipulation and analysis of DNA molecules with high specificity and precision.
Common Misspellings for BAMI ENDONUCLEASE
- vami endonuclease
- nami endonuclease
- hami endonuclease
- gami endonuclease
- bzmi endonuclease
- bsmi endonuclease
- bwmi endonuclease
- bqmi endonuclease
- bani endonuclease
- baki endonuclease
- baji endonuclease
- bamu endonuclease
- bamj endonuclease
- bamk endonuclease
- bamo endonuclease
- bam9 endonuclease
- bam8 endonuclease
- bami wndonuclease
- bami sndonuclease
- bami dndonuclease
Etymology of BAMI ENDONUCLEASE
The word "BamI Endonuclease" has its etymology rooted in the scientific nomenclature system for naming enzymes.
The prefix "Bam" is derived from the name of a bacterium called "Bacillus amyloliquefaciens". This bacterium was first discovered to produce the restriction enzyme BamHI in the early 1970s. The name of the bacterium was used to designate the enzyme produced by it.
The letter "I" following "Bam" indicates that it is a type I restriction endonuclease. Restriction enzymes are classified into different types based on their properties and biochemical mechanisms. In this case, BamHI belongs to Type I, which is characterized by its multisubunit composition and specific recognition sequences.
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